Indirect immuno-fluorescence assay and enzyme immuno-assay for massive screening of adult T cell leukemia associated antibody.
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چکیده
منابع مشابه
Detection of Antinuclear Antibody in Childhood Rheumatic Diseases by Immunofluorescence Assay and Enzyme Immuno Assay
Background: Anti-nuclear antibodies (ANAs) are specific antibodies directed against a variety of nuclear antigens. Indirect immunofluorecence (ANA-IFA) and Enzyme Immunoassay (ANA-EIA) are commonly used methods for ANA detection. The positivity of ANA-IFA using HEp-2 cell substrate is 90-100% in systemic rheumatic diseases. On the other hand positivity of ANA-EIA is found to be much lower in di...
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Bacterial surface proteins are involved in direct contact with host cells and in uptake of nutrients from the environment (1). For this reason, cellular localization can provide insights into the functional role of bacterial proteins. Surface localization of bacterial proteins is a key step towards identification of virulence factors involved in mechanisms of pathogenicity. Methods for fraction...
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Background: Potency evaluation of rabies vaccine is a cheap, fast, high precision and consistent with ethical values is critical, so researchers have modified a variety of methods such as: National Institute of Health (NIH) method, Single Radial Immunodiffusion (SRID) and so on. The purpose of the present study was to replace an in vitro method consistent with medical ethics criteria instead of...
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Background: The occurrence of autoimmune disorders has increased 3–5% and has had a significant impact on human health. The presence of Anti-Nuclear Antibodies (ANAs), directed against intracellular antigens, is a sign of systemic autoimmune rheumatic diseases. The Indirect Immunofluorescence Assay (IFA) is among the most commonly used methods for ANA detection and is the gold standard. An Immu...
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An attempt was made to standardize an in-house immuno-enzyme assay for measuring antibodies of infectious bursal disease virus (IBDV) in chicken. The test was performed after coating plates with ELISA antigens prepared by two methods. Antigen “A” was prepared from infectious bursal disease virus infected chicken embryo fibroblasts culture by concentration with dialysis against PEG-6000 while an...
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ژورنال
عنوان ژورنال: Journal of the Japan Society of Blood Transfusion
سال: 1984
ISSN: 1883-8383,0546-1448
DOI: 10.3925/jjtc1958.30.445